Govt. Degree College, Kodur(RS), Kadapa(Dist)
Affiliated to S.V. University, Tirupati.
THREE YEAR B.Sc. DEGREE EXAMINATIONS Pre Final 2012
Third Year Examination
Part II – Biotechnology
Paper Ill- Molecular Biology, Genetic Engineering & Immunology
Time: 3 hours Max Marks: 100
SECTION – I
Answer all questions. Each question carries 15 marks. (Marks: 4 x 15 60)
1. (a) Explain the mode of DNA replication with the help of Messelson & Stahl experiment.
(or)
(b) Explain the formation of TT dimmers and different types of repair mechanisms involved.
2. (a) Describe the mechanism of initiation and elongation of transcription
(or)
(b) Explain Gene regulation with the help of transcription
3. (a) Explain the different type of vectors used in r-DNA technology.
(or)
(b) Explain the different methods of introduction of r-DNA into host cells.
4. (a) Write an account on cells of the immune system
(or)
(b) What is Major histocompatibility complex. Explain its role in organ transplantation.
SECTION II
Answer any EIGHT questions. Each question carry 5 Marks. (Marks: 8 x 5 40)
5. Klenow fragment
6. Origin of replication
7. Telomeric DNA.
8. RNA Polymerase.
9. Termination of transcription.
10. Post transcriptional modifications
11. PCR.
12. Restriction endonucleases
13. Southern blotting.
14. Hapten.
15. Antigen Antibody reactions.
16 Hyper sensitivity
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