THREE YEAR B.Sc. DEGREE EXAMINATION, APRIL/MAY 2014.
THIRD YEAR EXAMINATION
Part II – Biotechnology
Paper III : MOLECULAR BIOLOGY, GENETIC ENGINEERING AND IMMUNOLOGY
Time: Three hours Maximum 100 marks
SECTION — I (4 x 15 = 60 marks)
Answer ALL questions.
1. (a) Describe the replication mechanism of DNA?
(or)
(b) Describe the structure of telomeric DNA and mention its significance
2. (a) Write a general account of gene expression
(or)
2. (a) Write a general account of gene expression
(b) Describe in detail about the features of genetic code
3. (a) Write a general account of vectors?
(or)
(b) Describe about the enzymes used in cloning?
4. (a) Explain hypersensitivity in detail ?
(or)
(b) Describe the structure and functions of immunoglobulins?
SECTION II – (8 x 5-40 marks)
Answer any EIGHT questions
5. Messelson and Stabl experiment
6. DNA damage
7. Nick translation
8. Wobble hypothesis
9. Structure of promoter
10. Significance of o (sigma) factor
11. cDNA-rDNA
12. PCR
13. Western blotting
14. Haptens
15. Agglutination
16. Antigen-Antibody reaction